molecular diagnostic markers is now being felt.This will grow as more molecular markers are validated and launched for thei r diagnostic and prognos tic v alue. by infectious disease testing (50-60%), with about another third attributable to genetic testing, and blood screening applications accounting for much of the rest. molecular methods include metagenomics sequencing mNGS (1) and RT-qPCR(2), both are excellent and sensitive techniques, but approaches not without limitations. Today, many conventional PCR methods are being replaced by real-time PCR, which allows more rapid detection and quantification of the PCR product, as well as detection of different strains of the pathogen by melting curve analysis. Join LinkedIn. - Molecular Diagnostics market is segmented by Type, and by Application. Specific chromosomal abnormalities and gene mutations may serve as diagnostic and prognostic indicators for disease progression and survival. This report focuses on the top players in North America, Europe, Asia-Pacific, South America, and Middle East & Africa. Other widely used molecular diagnostic techniques are isothermal amplification methods and nucleic acid hybridization techniques. Molecular diagnostic techniques - Dr Meghana P Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Microarray is a technique which holds promise and has an exceptional sensitivity and the capacity to detect several pathogens simultaneously. Whether your application is business, how-to, education, medicine, school, church, sales, marketing, online training or just for fun, PowerShow.com is a great resource. In many clinical settings, polymerase chain reaction (PCR) is clearly the method of choice due to its exquisite sensitivity and specificity. MOLECULAR DIAGNOSTIC METHODS cells form a mass that is detected by radiologic or gross pathologic examination5. 20-30 bp in length, Tm 10C higher than primer. - This report studies the global Molecular Diagnostics market status and forecast, categorizes the global Molecular Diagnostics market size (value & volume) by key players, type, application, and region. Diseases are the primary constrains for the development of any living forms i.e., both plants and animals including humans. China In–Vitro Diagnostics (IVD) Market, By Segments & Companies. Clinicians need to recognize the enhanced accuracy and speed of the molecular diagnostic techniques for the diagnosis of infections, but also to understand their limitations. presentations for free. Clinical microbiology laboratories increasingly rely on molecular diagnostic techniques. Malaria is caused by the parasite Plasmodium, The parasite infects and destroys red blood, Symptoms include fever, rashes and damage to, Current testing involves microscopic observations, A DNA diagnostic system would only measure, Find a probe that just hybrisized with Plasmodium, The probe is able to detect 10 pg of purified DNA, Other DNA probes were developed for the following. Taking advantage of the many major advances that have occurred since their groundbreaking first edition was published, William B. Coleman and Gregory J. Tsongalis have updated and expanded their highly praised tutorial guide to molecular diagnostic techniques to include not only improved traditional methods, but also totally new molecular technologies, some not yet in routine use. Molecular Diagnostics is a discussion forum and platform for Molecular Biologists in Belgium affiliated to hospital laboratories and active in the field of Molecular Diagnostics. ... Our one-step syndromic real-time PCR products simultaneously detect viruses, bacteria, fungi, and parasites, allowing molecular laboratories to lower cost and drive better outcomes. Moleculardiagnostics.be. Laboratory results should always be interpreted in the context of the clinical presentation of the patient, and appropriate site, quality, and timing of specimen collection are required for reliable test results. China Molecular Diagnostics Market & Volume Forecast, by Application. Most commonly used methods are protein methods, immunostaining methods, nucleic acid methods. Periodontitis: is characterized by a loss of connective tissue attachment that begins at. If so, share your PPT presentation slides online with PowerShow.com. - According to MRFR Analysis – Sepsis Diagnostics Market is growing at a CAGR of 8% during the forecast period 2016 to 2022. ... a huge step forward over existing techniques, which are complex, expensive and take several days. A detailed description of advanced dignostic aids in periodontics. Do you have PowerPoint slides to share? Naturally occurring variations in the nucleotide sequence of DNA results in … Molecular technology • Molecular diagnosis is the most appropriate for infectious agents that are difficult to detect, identify, or test for susceptibility in a timely fashion with conventional mehtods. Molecular Forensics offers a comprehensive coverage of the increasingly important role that molecular analysis plays with in forensic science.Genotyping with DNA probes can theoretically identify each person on earth. Advanced diagnostic techniques in periodontics pdf - Advanced Diagnostic Aids Dr V. M. Joshi. Even as many restriction enzymes are known, some mutation sites do not correspond to any, RAPD is often used to show relatedness among DNA, In this procedure arbitrary (random) primers are, A single primer is used which must anneal in 2, The primers (8-10nt) are likely to anneal in many, Amplified products are separated by agarose gel, If the samples have similar genetic make up then, This procedure is widely used to differentiate, Issues to consider when using this procedure, Many diseases are caused by a single nucleotide, A single nt change can be detected by PCR/OLA, Bacterial sensors can be used to test for, Bacteria with bioluminescent are good candidates, In the presence of pollutants the bioluminescent, The structural genes (luxCDABD) encodes the, The cells that luminescence to the greatest, To screen water samples for pollutants (metal or, After a 15 min incubation the luminescence of the, When the solution contained low to moderate, The procedure is rapid, simple, cheap and a good. By continuing you agree to the use of cookies. Actually, the best approach for managing pa-tients with breast implant associated ALCL is controversial. DNA fingerprinting (DNA typing) is used to, -gt In legal proceedings to identify suspects and, Former used with southern blot experiments. The application of molecular biology and genetic techniques has greatly contributed to recent advances in hematology. The global market industry segmented by Technology, Type of Product, End User, And Region. Players, stakeholders, and other participants in the global Molecular Diagnostics market will be able to gain the upper hand as they use the report as a powerful resource. Call Us : +1 678-302-0700, Sepsis Diagnostics Market Segmentation | by Technology, Type of Product, End User, and Region| Forecast – 2022. It is expected to grow at a compound annual growth rate (CAGR) of 8.82% and reach $12.09 billion by 2023. Dec 10, 2020 - Molecular Diagnostics, PPT (Powerpoint Presentation), Semester, Engineering NEET Notes | EduRev is made by best teachers of NEET. Human Chromosomes by Miller & Tharman, Springer Publishing Company, 9. Hospitals and diagnostic laboratories using cellular and molecular diagnostic techniques need products that guarantee the highest levels of reliability and the greatest speed. Molecular Diagnostics Market Size & Share [2018-2023], - The molecular diagnostics market is projected to reach USD 11.54 Billion by 2023 from USD 7.10 Billion in 2017, at a CAGR of 8.4%. Get a Free Sample Report. Or use it to upload your own PowerPoint slides so you can share them with your teachers, class, students, bosses, employees, customers, potential investors or the world. Elsevier’s Novel Coronavirus Information Center, https://doi.org/10.1016/j.mpmed.2009.07.012. It's FREE! Knowledge, Well Presented Sign up for a LinkedIn account to learn or share your insights about any topic on SlideShare. 3. Molecular methods also offer faster turnaround time and do not require large sample volumes. That's all free as well! Call Us : +1 678-302-0700 Follow the Link for More Information: https://www.renub.com/molecular-diagnostics-market-nd.php, - The global malaria diagnostics market is expected to register a significant growth of 4.6 % CAGR during the forecast period (2018–2023), Global Molecular Point of Care Diagnostics Market. Winner of the Standing Ovation Award for “Best PowerPoint Templates” from Presentations Magazine. Over the past century microbiologists have searched for more rapid and efficient means of microbial identification. Allow to detect alternative spliced isoforms. - The study segments Molecular Diagnostics industry in light of major classification such as product type, potential markets, application, and end-user. Global Molecular Diagnostics Market will be USD 22 Billion by 2025 - Renub Research, - Global Molecular Diagnostics Market is anticipated to cross USD 22 Billion by the end of year 2025. Molecular techniques also enable clinicians to monitor disease progression during treatment and to make accurate prognoses. Expression proteomics:- It refers to the quantitative study of protein expression between sample differing by some variable. The various formats of nucleic acid amplification are the most frequently used molecular tests in the diagnosis of infectious diseases. Full report: http://kbvresearch.com/global-molecular-diagnostics-market/, Japan In Vitro Diagnostics (IVD) Market Share & Forecast By Diagnostics - Renub Research, - According to Renub Research analysis Japan In-vitro Diagnostic Market is projected to reach US$ 4.43 Billion by 2026. The ability to measure the quantity of microbe by quantitative PCR has become increasingly important, providing information on the progression and prognosis of disease, and effectiveness of treatment. Published by Elsevier Ltd All rights reserved. The PowerPoint PPT presentation: "MOLECULAR DIAGNOSTICS" is the property of its rightful owner. - Get more details @ http://bit.ly/2ePRhAo Some of the players in global molecular diagnostics market are Abbott Molecular, BioMerieux’s, Cepheid, Danaher Corporation, Qiagen Gaithersburg, Roche diagnostics, and Siemens Healthcare. After you enable Flash, refresh this page and the presentation should play. 35-65 G/C more Cs than Gs. are not culturable. Join SlideShare to discover, share, and present presentations and infographics on the world’s largest professional content sharing community. Forecast by Segment (Clinical Chemistry, Immunoassay, Molecular Testing, Microbiology, Hematology, SMBG, Point of Care Testing, Coagulation), Company Analysis. Broadening information amidst the doctors and inmates concerning the advantages and availability of equipment will assist in encouraging the growth of the market. Advances in periodontal diagnosis, in the assessment of gingival inflammation, in the. mNGS is restricted by throughput, turnover time, formidable high cost and requirement for high technical expertise. Read More @ https://www.thebusinessresearchcompany.com/report/molecular-diagnostics-market-global-report-2020-covid-19-implications-and-growth, Molecular Diagnostics Market 2020 Global Share, Future Growth, Demand and Forecasts to 2025. By Application (2019 - 2025). Newer molecular techniques such as the polymerase chain reaction (PCR), ligase chain reaction (LCR), nucleic acid based amplification (NASBA), and branched DNA (bDNA) depend on some form of amplification, either the target nucleic acid, or the signal itself. Microarray is a technique which holds promise and has an exceptional sensitivity and the capacity to detect several pathogens simultaneously. Analysis of hormones, vitamins, metabolites. - China In Vitro Diagnostics Market will be USD 22.3 Billion by 2026. An introduction to Human Molecular Genetics by Pasternak et al., John Wiley & Sons 8. Molecular virology refers to the specific detection of viral infection utilizing molecular detection methods. - The global market size of Point of Care (PoC) Molecular Diagnostics is $XX million in 2018 with XX CAGR from 2014 to 2018, and it is expected to reach $XX million by the end of 2024 with a CAGR of XX% from 2019 to 2024. Forecast For By Diagnostics & Reimbursements. Molecular techniques have increasingly been used in plant diversity conservation and isolation of PCR amplifiable genomic DNA is an important pre-requisite. - China Molecular Diagnostics Market is expected to be US$ 6.3 Billion by 2026. Eg. MOLECULAR HYBRIDIZATION TECHNIQUES OF NUCLEIC ACIDS Vasilica BARBU * “Dunarea de Jos” University, Faculty of Food Science and Engineering, 111 Domnească St., 800201, Galaţi, Romania Abstract: The nucleic acid hybridization is the process wherein … - The Global Molecular Diagnostics Market is expected to attain a market size of $11.2 billion by 2022, growing at a CAGR of 8.6% during the forecast period. Advances in molecular biology over the past 10 years have opened new avenues for microbial identification and characterization (1)(2)(3)(4)(5). He has published and presented papers relating to antimicrobial resistance, respiratory pathogens, molecular diagnostics and infection control. Cell and Molecular Biology by De Robertis and De Robertis, Lipincott &Wilkins 12. Annuleren Remove. Data Bridge Market Research analyses the market to growing at a CAGR of 5.30% in the above mentioned forecast period. As with most molecular diagnostics, RT-qPCR is the most - Viral Molecular Diagnostics market is segmented by Type, and by Application. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. They are all artistically enhanced with visually stunning color, shadow and lighting effects. Report and Proceedings of the Expert Workshop on DNA-based Molecular Diagnostic Techniques: Research Needs for Standardization and Validation of the Detection of Aquatic Animal Pathogens and Diseases. Other widely used molecular diagnostic techniques are isothermal amplification methods and nucleic acid hybridization techniques. And they’re ready for you to use in your PowerPoint presentations the moment you need them. Molecular Diagnostics Market to witness more than 8.4% CAGR from 2017 to 2024. Reliability is essential, because an inaccurate or missed diagnosis can be a matter of life and death. - Global Transplant Diagnostics Market size is projected to be valued $XX Billion by 2024; with a CAGR of XX% from 2017 to 2025. Molecular Diagnostics Market Industry Trends And Emerging Opportunities Till 2023, - The global molecular diagnostics market was worth $8.62 billion in 2019. -gt can be used for quasi High-throughput!!! Many molecular diagnostics techniques or tests first developed for their use in molecular biology and biotechnology laboratories are now routinely used in clinical laboratories. Bangkok, Thailand, 7-9 February 1999. However, microarrays are currently too expensive to be adapted for routine diagnostics, and their diagnostic use requires broad-based nucleic acid amplification prior to analysis which is not well established. Molecular Diagnostics Tests & Reagents. Volume Forecast, by Application (Oncology Testing [Breast Cancer, Colorectal Cancer, Prostate Cancer), Infectious Disease, and Genetic Disease Testing). The traditional methods of microbial identification rely solely on the phenotypic characteristics of the organism. A review of management modal-ities applied to these patients could help in elu-cidating appropriate management such as longer This document is highly rated by NEET students and has been viewed 783 times. currently, molecular diagnostic revenue is dominated Figure 2: Molecular diagnostics as a component in the global in vitro diagnostics market. - The molecular point of care diagnostics market is estimated to grow at a CAGR of 14.05% and expected to reach $7398.82 million by 2028. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Health information and medical research on Novel Coronavirus (2019-nCoV) are available at. Many of them are also animated. Many new technologies have found utility in the clinical routine. | PowerPoint PPT presentation | free to view, Molecular Diagnostics Market Analysis | CMI, - Read here the latest updates on the Molecular Diagnostics Market published by CMI team, Global Viral Molecular Diagnostics Market Size, Status and Forecast 2020-2026. 2. By 2024, Molecular Diagnostics Market in Europe to hit $3.3 Bn : Grapghical Research. These genes (DNA) can be used as diagnostic tools. Molecular diagnostics is a collection of techniques used to analyse biological markers in the genome and proteome—the individual's genetic code and how their c… Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Players, stakeholders, and other participants in the global Viral Molecular Diagnostics market will be able to gain the upper hand as they use the report as a powerful resource. Molecular diagnostics is a collection of techniques used to analyse biological markers in the genome and proteome—the individual's genetic code and how their cells express their genes as proteins—by applying molecular biology to medical testing.The technique is used to diagnose and monitor disease, detect risk, and decide which therapies will work best for individual patients. Oncology Molecular Diagnostics Market Research Report, Global Insights And Forecast By 2023, - TBRC’s research report focuses on key targets and business needs, in order to achieve strong sales in the Global Oncology Molecular Diagnostics Market. - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. And, best of all, most of its cool features are free and easy to use. In diagnostic clinical virology, there has been a wholesale move from culture to polymerase chain reaction (PCR) and, more recently, sequencing. Molecular Diagnostics DNA Diagnostic Systems Hybridization 31 TaqMan Probe design Molecular Diagnostics DNA Diagnostic Systems Hybridization. 7. Or use it to find and download high-quality how-to PowerPoint ppt presentations with illustrated or animated slides that will teach you how to do something new, also for free. https://bit.ly/3o3LBmy, Global Molecular Diagnostics Market Size, Status and Forecast 2020-2026. ACTH, FSH, T3, T4, Glucagon, Insulin, Diagnostic procedures for detecting infection, involving various non-covalent interactions. diagnostic clinical microbiology, and is currently leading a project to develop new diagnostic platforms for infectious diseases. Or use it to create really cool photo slideshows - with 2D and 3D transitions, animation, and your choice of music - that you can share with your Facebook friends or Google+ circles. We use cookies to help provide and enhance our service and tailor content and ads. It is increasingly evident that molecular diagnostics, that is, the use of diagnostic testing to understand the molecular mechanisms of an individual patient’s disease, will be pivotal in the delivery of safe and effective therapy for many diseases in the future. Our new CrystalGraphics Chart and Diagram Slides for PowerPoint is a collection of over 1000 impressively designed data-driven chart and editable diagram s guaranteed to impress any audience. bDNA is essentially a conventional hybridization technique with increased sensitivity. When the probe and primer anneal to the target, Test that primer and probe are not complementary, Tm of probe region should be 7-10C above target, Check that there is no complementarity between, Tm of probe alone and probe complement should, Age related aneuploidy (abnormal number of, All cells uniformly abnormal eg trisomy 21, often diploid with aneuploid or tetraploid cells, Different chromosome pattern in every cell, Translocations (rearrangement of parts between, Older women likely to produce abnormal oocytes, recurrent IVF ( in vitro fertilization) failure, X-linked and affect mainly males an estimated 1, strengthen muscle cells by anchoring elements of, Mutated dystrophin leads to implosion of muscle, Specific primers are used to amplify a 156 bp, Using standards the amount of PCR product can be, PCR can also be used as a prognostic tool to, This method can also be used to determine the, RFLP Restriction Fragment Length Polymorphism, Regular fingerprinting analyses phenotypic. Several molecular methods can be used for genotyping, which allows the identification of different subtypes of the pathogen; genotyping plays a role in the risk assessment and management of infections. The growing awareness regarding the testing methods among patients has been directly impacting the growth of point of care molecular diagnostics market. DNA fingerprinting analyses genotypic traits. Delete Bent u zeker dat u dit item permanent wilt verwijderen? Molecular Diagnostics Market Share & Global Forecast, By Application, - According to Renub Research analysis Global Molecular Diagnostics Market is likely to surpass US$ 22.5 Billion by the end of year 2025. - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. PPT – MOLECULAR DIAGNOSTICS PowerPoint presentation | free to view - id: 3c01e9-OThkY, The Adobe Flash plugin is needed to view this content. Copyright © 2009 Elsevier Ltd. 7. - Graphical Research has reported the addition of the “Molecular Diagnostics Market: Europe Industry Analysis and Opportunity Assessment 2018 - 2024" report to their offering. The identification of these anomalies by state-of-the-art molecular (cyto)genetic techniques such as fluorescence in situ hybridization (FISH), comparative ge … The identification and differentiation of microorganisms has principally relied on microbial morphology and growth variables. Blood sample (most common for adult testing), Mouthwashes or buccal scrapes (non-invasive), Chorionic villus biopsy samples (fetal DNA), One or two cells removed from 8-cell embryo (in, Archived pathological specimens (typing dead, Bacterial and viral pathogens may be pathogenic, Genetic diseases often are due to mutations or. Access full Research: https://www.renub.com/global-molecular-diagnostics-market-and-future-forecast-2010-2014-334-p.php, Global Point of Care (PoC) Molecular Diagnostics Market Report 2019 - Market Size, Share, Price, Trend and Forecast. 20-30 bp in length, Tm 10C higher than primer. - The global molecular diagnostics market is projected to grow with rising technological developments in molecular diagnostic techniques. Molecular diagnostic testing combines laboratory testing with the precision of molecular biology and has revolutionized the way clinical and public health laboratories investigate the human, viral, and microbial genomes, their genes, and the products they encode. He … Molecular Biology of the cell by Alberts et al., Garland Press 10. Full Report: https://kbvresearch.com/global-molecular-diagnostics-market/, Global Molecular Diagnostics Devices And Equipment Market 2019, - A report from TheBusinessResearchCompany shows that the "Global Molecular Diagnostics Devices And Equipment Market 2019" is expected to grow to $7.71 billion at a CAGR of 0.7% through 2022. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. Read More https://www.marketsandmarkets.com/Market-Reports/molecular-diagnostic-market-833.html, - The Global Molecular Diagnostics Market is expected to attain a market size of $11.2 billion by 2022, growing at a CAGR of 8.6% during the forecast period. To view this presentation, you'll need to allow Flash. Molecular Biology methods used to study the molecular basis of biological activity. Point of Care Molecular Diagnostics Market Growth Analysis, Market Scope And Forecast by 2027. Global Forecast by Application, Technology, End User, Regions, Companies. - Point of care molecular diagnostics market is expected to reach a market value of USD 5.12 billion by 2027 growing at a CAGR of 12.9% in the forecast period of 2020-2027. If you continue browsing the site, you agree to the use of cookies on this website. Transplantation is the process of relocating organ in order to treat organ failure such as, Noninvasive Cancer Diagnostics Market-Global Industry Analysis and Forecast to 2020 to 2027. Molecular techniques 1. The segmental analysis focuses on revenue and forecast by Type and by Application in terms of revenue and forecast for the period 2015-2026. Laboratory Diagnostic Solutions India. CrystalGraphics 3D Character Slides for PowerPoint, - CrystalGraphics 3D Character Slides for PowerPoint. Back to MLAB 2378 Fundamentals of Molecular Diagnostics Back to MLAB 2479 Molecular Diagnostics Techniques NOTE: Some links below are listed twice in different areas to more easily find specific information Remember: Right click on the link to open in a new window. PowerShow.com is a leading presentation/slideshow sharing website. Bacterial fermentati… Because such molecular-based testing enables performance of diagnostics with noninfectious inactivated pathogens, the need for costly and complex BSL-3 or -4 containment is obviated for diagnostic work on very hazardous pathogens. 35-65 G/C more Cs than Gs. - Noninvasive cancer diagnostics market is expected to gain market growth in the forecast period of 2020 to 2027. Detection based on enzyme catalyzed reactions, Detection based on fluorescent labeled secondary, An immunoelectronmicrograph of the surface of a, (The density of class I exceeds that of class II), Electron-dense label (ferritin or colloidal, Does THIS patient have ANY mutation in ANY gene, Does THIS patient have ANY mutation in THIS gene, Does THIS patient have a 3-bp deletion of Phe, RNA RT-PCR allow to test genes directly, without. Read more at https://bit.ly/2kvoUvV, Global Molecular Diagnostics Market Research Report 2018. Genes IX, by Lewin B, Pearson India 11. These methods used to explore cells, their characteristics, parts, and chemical processes, and pays special attention to how molecules control a cell’s activities and growth. Functional proteomics:- It refers to the use of proteomics techniques to analyze the characteristics of molecular protein-networks involved in a living cell. Introduction Definitions, Problematics, Examples, The success of modern medicine depends on the, A good detection system should have 3 qualities, Sensitivity means that the test must be able to, Specificity the test yields a positive result, High throughput testing for genetic disorders, Progress is being made in all of these areas, Each of these areas are commercially attractive, In some cases, the MassARRAY platform is uniquely, More tests will be added to the platform as these, Non-invasive, early detection of organ rejection, Example urine testing for kidney rejection, Pathogen identification and early detection, Examples identification of multi drug resistant, 130 million live births worldwide per year, 8 million live births in US and Europe per year, 6 of all babies are born with birth defects, Down syndrome is the most common chromosomal, Although risk increases with age, 80 of Down, Even though limited to high risk mothers, still a, single base extension of these primers with, -gt each base is identified by 2 unique 25-mer. Copyright © 2020 Elsevier B.V. or its licensors or contributors. Can try as high as. Molecular Diagnosis Dr.M.Malathi Postgraduate II year Department of Microbiology Chengalpattu medical college 2. Is similar to an enzyme-substrate interactions, Strength of Antigen-Antibody Interactions, Enzyme-Linked ImmunoSorbent Assay (ELISA), Alternatives to Antigen-Antibody Reactions, is a quantitative measure of binding strength, Antibody avidity (describes the binding intensity, True strength of the Ab-Ag interaction within, The interaction at one site will increase the, High avidity can compensate for low affinity, (IgM may have low affinity but it has high, High affinity complexes have high Ka values, Very stable complexes have very low values of Kd, Antibody elicited by one Ag can cross-react with, occurs if two different Ags share identical or, Addition of secondary antibody which will bind, The secondary antibody usually has attached to it, Addition of a colourless substrate which will. Recent advances in the disgnosis of periodontal diseases including advances in the assessment of gingival inflammation, advances in the assessment of loss of periodontal attachment, advances in radiographic diagnosis, advances in microbiological analysis, Advances in immunodiagnostic techniques, advances in assays based on molecular biology techniques, advances in identifying … Can try as high as 80 or as low as 20 if the region is particularly GC or AT rich.
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